Real-time RT-PCR in COVID-19 detection: issues affecting the results

Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

Factors Influencing Real-Time RT-PCR Results: Application of Real-Time RT-PCR for the Detection of Leukemia Translocations

A variety of factors that influence real-time reverse transcriptase (RT)-PCR results were examined. Realtime reaction volumes can be decreased from the manufacturers suggested volumes (50μl) to as little as 5μl. Errors introduced through volume handlings can be significantly mitigated through the use of electronic pipettes. Random hexamers are compared against the use of a specific reverse tran...

COVID-19 in Nursing Homes: The Problematic Management of Residents Without Positive COVID-19 RT-PCR

Appling real time RT-PCR for bluetongue virus detection in Iran

During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in 310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conserve genes to search any BTV strains. Using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. Sensitivity of both molecular techniques evalua...

Detection of Circulating Tumor Cells in Breast Cancer Patients Using Cytokeratin-19 Real-Time RT-PCR

PURPOSE The roles of circulating tumor cells (CTCs) as predictive and prognostic factors, as well as key mediators in the metastatic cascade, have been investigated. This study aimed to validate a method to quantify CTCs in peripheral blood using a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay for cytokeratin (CK)-19 and to evaluate the utility of this assay in detect...